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  • Genome comparison: Perform comparative genomics of two mammals using VISTA suite as well as to study microbial genome annotation and comparison using IMG Metagenome Analysis System.

  • Phylogenetic Reconstruction: Study the phylogenetic relationship among various bacterial species using "Phylogeny.fr" and analyse phylogenetic relationship among various mammalian species using T-Rex Tree and reticulogram REConstruction.

  • Identification of proteins using database searches : Identify proteins on the basis of sequence conservation pattern using database searches PROSITE,also to identify proteins on the basis of domain decomposition using Pfam.

  • Predicting Protein-Protein interactions: Study annotated and reviewed Protein-Protein interactions using MIPS and predict Protein-Protein interactions and Quaternary structure using CluPro.

  • Primer designing: Design Primers for PCR using Primer BLAST and for selective amplification of genes using Primer3.

  • Handle Sequence database & alignment tools: Use various sequence database like GenBank, UniProt-KB/SwissProt, DOTTUP, NEDDLE, WATER, ClustalW. Perform various Protein Structure Prediction Tool/Software like SwissModel, GOR (Garnier Osguthorpe-Robson) method, JPred (Jnet Prediction) and carry out Sequence Based Database Search and Gene prediction using BLAST (Basic Local Alignment Search Tool)

  • Immunological assays: Perform radial immunodiffusion,double immunodiffusion (Ouchterlony ) assay,determine total leukocyte count,determine blood group of own blood,separate serum from blood,determine differential leukocyte count perform the isolation of lymphocyte population in blood sample,estimate Hb content in blood with Sahli's and Drabkin's method,perform DOT-ELISA.Agglutination methods and perform latex agglutination to determine Ag-Ab interaction.

  • Molecular biologal assays: Isolate DNA from Plant & Bacteria,Isolate Plasmid DNA from bacteria,Isolate genomic DNA and RNA from E.coli cells,perfrom Agarose gel electrophoresis of DNA samples,Spectrophotometric analysis of DNA,Gene amplification by PCR,Restriction digestion of given DNA samples,Develop antibiotic resistance in bacteria due to transformation and perform Heat-Shock method of transformation.

  • Microbial metabolism & sugar utilization tests: Carry out starch hydrolysis,casein hydrolysis,indole test,methyl red test,voges -proskauer test,citrate test,catalase test,urease test,lactose fermentation test and Motility by stab technique.Isolate Lactic Acid Bacteria from Curd and estimate Total Plate Count in any food sample.

  • Biochemical & Organic experiments: Perform qualitative tests for carbohydrates which includes tests for reducing and non reducing sugars,aldoses and ketoses.Qualitative tests for amino acids and proteins,determine presence of carbohydrate by dubois method, determine proteins by lowry method,determine acid value of fat,carry different techniques of distillation,crystallization and sublimation techniques.

  • Microbial & tissue culture experiments: Carry out sterilization of various glasswares, media preparation by autoclaving and isolate microorganisms from soil,water,air by pour plate, spread plate and streak plate method,Preparation of DMEM and sterilization by membrane filter, Prepare RPMI1640 and sterilize by membrane filter,trypsinization of adherent cell culture, counting of cells using haemocytometer,estimation of viability by dye exclusion,Culturing of cell line for 70-80% confluency,Isolation & culturing of Peripheral Blood Mononuclear cell,surface sterilization,perform immobilization of plant cells,in-vitro germination of seeds,perform explant preparation,organ culture,perform initiation & induction of callus cultures,prepare and inoculate explant for micropropagation(Shoot tip culture).

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